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Lumonics Inc frequency-doubled ruby laser
Frequency Doubled Ruby Laser, supplied by Lumonics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/frequency-doubled+ruby+laser/frequency+doubled+ruby+laser/pmc10235942-146-9-20
Average 90 stars, based on 1 article reviews
frequency-doubled ruby laser - by Bioz Stars, 2026-11
90/100 stars

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Article Title: Contractile properties and proteins of smooth muscles of a calponin knockout mouse
Article Snippet: Briefly, α-toxin-permeabilized bundles of bladder were transferred from G1 (Ca 2+ -free, 1 m m EGTA) to a 25 μl trough containing pCa 6.0 solution containing caged GTPγS (200 μ m ) and glutathione (3 m m ) for 5 min. Caged GTPγS was photolysed using a frequency-doubled ruby laser (Lumonics, Warwick, UK), delivering a 50 ns pulse of near-UV light at 347 nm, sufficient to release 14 ± 2.3 μ m GTPγS ( n = 6, where n refers to the number of experimental observations), as determined by HPLC of the trough solution after completion of the force measurements.

Article Title: Resolving the Fast Kinetics of Cooperative Binding: Ca 2+ Buffering by Calretinin
Article Snippet: Briefly, it consisted of a small chamber (20 μl) mounted on an inverted microscope equipped for epifluorescence (IM35; Carl Zeiss) with a 505-nm dichroic mirror and 510 LP emission filter (Chroma Technology).

Article Title: The ATP hydrolysis and phosphate release steps control the time course of force development in rabbit skeletal muscle
Article Snippet: The 8 mm diameter beam from a frequency-doubled ruby laser (347 nm: Lumonics QSR2/6) was focused down with a cylindrical lens (UL2: focal length 200 mm) in the vertical direction to a height of about 1 mm at the fibre and masked off in the horizontal direction so that the fibre plus about 200 μm of the aluminium T-clip holding each end of the fibre was illuminated.

Article Title: Kinetic Properties of DM-Nitrophen Binding to Calcium and Magnesium
Article Snippet: Briefly, it consisted of a small chamber (20 μ l) mounted on an inverted microscope equipped for epifluorescence (IM35; Zeiss, Oberkochen, Germany) with a 505-nm dichroic mirror and 510 LP emission filter (Chroma Technology, Rockingham, VT).

Article Title: The effects of the inotropic agent EMD 57033 on activation and relaxation kinetics in frog skinned skeletal muscle.
Article Snippet: The effect of the cardiotonic sensitizing drug EMD 57033 was studied in frog skinned skeletal muscle fibres at 12°C to provide a baseline for skeletal muscle studies and for comparison with cardiac fibres.. The activation and relaxation of fibres were induced by laser flash photolysis of the caged calcium NP-EGTA, and caged calcium chelator diazo-2 respectively.. EMD 57033 (10 μM) slightly increased the rate of relaxation (rate constant k1 changing from 24.0±2.9 s–1 in control to 28.1±3.2 s–1) but had no significant effect on the rate of activation (k1=9.6±0.9 s–1 in control conditions, 9.7±1.6 s–1 with EMD 57033).

Article Title: Activation of skeletal muscle is controlled by a dual-filament mechano-sensing mechanism
Article Snippet: For experiments with caged calcium (NP-EGTA, N6802, Invitrogen), a UV pulse from a frequency-doubled ruby laser (λ = 347 nm, Lumonics Ltd, UK) circularly polarized with a λ/4 waveplate was attenuated via a quartz beamsplitter and microscope slide attenuators to ~60 mJ and vertically focused on the fiber with a cylindrical lens.

Article Title: Myosin essential light chain isoforms modulate the velocity of shortening propelled by nonphosphorylated cross-bridges.
Article Snippet: This was replaced by photolysis solution containing 5 mM caged ATP (1-(2-nitrophenyl)-ethyl-ATP; Molecular Probes, Eugene, OR), and 40 mM DTT, in zero Ca21-rigor solution (pH 7.1, ionic strength 0.16) for 3 min. Caged ATP was photolyzed using a frequency-doubled ruby laser (Lumonics, Warwick, United Kingdom), delivering a 50-ns pulse of near-UV light at 347 nm, sufficient to release 1.2 6 0.1 mM ATP (n 5 5), as determined by high performance liquid chromatography of the trough solution at the completion of force measurements.



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